Mortality of shrimp larvae test performed to determine the toxicity values propolis extract obtained values of 50% Lethal Concentration (LC50) at 249.6079 ppm for ethanol fraction as the most active fraction. Ethyl acetate fraction containing alkaloid compounds. Ethanol fraction containing alkaloids and phenolic compounds. Based on the test results of phytochemical screening of secondary metabolites contained in propolis extracts showed that the crude extract contains phenolic compounds and alkaloids. Phytochemical test, test the mortality of larvae shrimp (Brine Shrimp Lethality Test) and test the antioxidant activity of secondary metabolites extract of propolis Trigona Incisa been done.
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